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The Medium of the Culture Tissue?

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  • The Medium of the Culture Tissue?

    Hi everyone,

    I am newbie here.

    I do not know exactly which one is the true term, the culture tissue or the tissue culture.

    I would like to ask a question.

    What is the medium (liquid?) is used in the tissue culture method such as below image?

    http://www.molbi.de/material_stuff/p...lture_jars.jpg

    Best regards

    Tece

  • #2
    If you'll be more specific about what you're trying to do and where you are located, I might click on your link.

    But I don't like clicking on random links posted on websites, if I don't know the person who posted it.

    .

    Comment


    • #3
      I think it is agar.
      Mark

      Vegetable Kingdom blog

      Comment


      • #4
        Originally posted by Capsid View Post
        I think it is agar.
        Well, I haven't looked at the link (waiting for the OP to clarify what they're up to), but if it's agar, I've got vast experience: I've made and used many tens of thousands of such culture media in the years when I worked as an infectious diseases scientist.
        .

        Comment


        • #5
          FB,the link goes directly to this image ..............

          He who smiles in the face of adversity,has already decided who to blame

          Artificial intelligence is no match for natural stupidity

          Comment


          • #6
            Originally posted by FB. View Post
            If you'll be more specific about what you're trying to do and where you are located, I might click on your link.

            But I don't like clicking on random links posted on websites, if I don't know the person who posted it.

            Please see the attached image.
            Attached Files

            Comment


            • #7
              So, if I would like to try the culture tissue method, all I have to do is just buy the agar then immerse the seed in it?

              Comment


              • #8
                Surely if you are doing a tissue culture you are growing a new plant from existing plant material - not a seed at all?
                Whooops - now what are the dogs getting up to?

                Comment


                • #9
                  tece,while you are waiting for FB,have a read of this ................

                  Preparing Plant Tissue Culture Medium
                  He who smiles in the face of adversity,has already decided who to blame

                  Artificial intelligence is no match for natural stupidity

                  Comment


                  • #10
                    Originally posted by tece View Post
                    So, if I would like to try the culture tissue method, all I have to do is just buy the agar then immerse the seed in it?
                    The agar will require various nutrients, which would normally be supplied by the roots.

                    It'll probably also require some antibiotics and antifungals (we used to use Fluconazol, Ceftazidime and Vancomycin, although some organisms now have evolved resistance to these) to prevent micro-organisms growing in or on it.
                    If you're not careful and don't use anti-microbials, your nutrient-enriched agar might end up growing all manner of bacteria and fungi; including some unpleasant micro-organisms which could make you ill. Fungal spores can cause respiratory irritation or induce asthma attacks and there's even a small chance that you might end up accidentally culturing some Bacillus anthracis (ANTHRAX) which is a soil-borne organism, or Clostridium tetani (TETANUS).
                    Last edited by FB.; 03-07-2012, 05:21 PM.
                    .

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                    • #11
                      He he..... having mentioned a dangerous micro-organism, my internet connection has gone weird.
                      Probably Her Majesty's Secret Services now watching me.

                      To save HMSS from having to waste too much time, my registration number on the Health Professions Council website is BS40157 (Biomedical Scientist).

                      Register can be checked here:
                      http://www.hpcheck.org/

                      Nothing sinister here. Keep calm and carry on!

                      Last edited by FB.; 03-07-2012, 05:38 PM.
                      .

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                      • #12
                        I wonder what tece is trying to grow and where?
                        Whooops - now what are the dogs getting up to?

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                        • #13
                          Originally posted by Jeanied View Post
                          I wonder what tece is trying to grow and where?

                          I understand that some rootstocks - such as M116 - are propagated in this way, as it's quicker than stooling/layering if the rootstock is a bit slow to root.

                          (slow rooting and more difficult propagation is why M116 never found success until lots of the commercial MM106's began to die from root diseases; a replacement rootstock with better disease resistance was urgently needed and M116 was too slow to propagate in the traditional way).

                          .
                          Last edited by FB.; 03-07-2012, 06:32 PM.
                          .

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                          • #14
                            Here you go - a link to Frank P Matthews website, which mentions M116:

                            Rootstocks : Background and History - Trees for Life - Frank P. Matthews Ltd.
                            .

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                            • #15
                              Originally posted by FB. View Post
                              To save HMSS from having to waste too much time, my registration number on the Health Professions Council website is BS40157 (Biomedical Scientist).
                              SWMBO is also on there FB & is also a Biomedical Scientist
                              He who smiles in the face of adversity,has already decided who to blame

                              Artificial intelligence is no match for natural stupidity

                              Comment

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