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Thread: The Medium of the Culture Tissue?

  1. #9
    bearded bloke's Avatar
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    tece,while you are waiting for FB,have a read of this ................

    Preparing Plant Tissue Culture Medium
    He who smiles in the face of adversity,has already decided who to blame

    Artificial intelligence is no match for natural stupidity

    Merry Christmad everybody

  2. #10
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    Quote Originally Posted by tece View Post
    So, if I would like to try the culture tissue method, all I have to do is just buy the agar then immerse the seed in it?
    The agar will require various nutrients, which would normally be supplied by the roots.

    It'll probably also require some antibiotics and antifungals (we used to use Fluconazol, Ceftazidime and Vancomycin, although some organisms now have evolved resistance to these) to prevent micro-organisms growing in or on it.
    If you're not careful and don't use anti-microbials, your nutrient-enriched agar might end up growing all manner of bacteria and fungi; including some unpleasant micro-organisms which could make you ill. Fungal spores can cause respiratory irritation or induce asthma attacks and there's even a small chance that you might end up accidentally culturing some Bacillus anthracis (ANTHRAX) which is a soil-borne organism, or Clostridium tetani (TETANUS).
    Last edited by FB.; 03-07-2012 at 06:21 PM.
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    He he..... having mentioned a dangerous micro-organism, my internet connection has gone weird.
    Probably Her Majesty's Secret Services now watching me.

    To save HMSS from having to waste too much time, my registration number on the Health Professions Council website is BS40157 (Biomedical Scientist).

    Register can be checked here:
    http://www.hpcheck.org/

    Nothing sinister here. Keep calm and carry on!

    Last edited by FB.; 03-07-2012 at 06:38 PM.
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  4. #12
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    I wonder what tece is trying to grow and where?
    Whooops - now what are the dogs getting up to?

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    Quote Originally Posted by Jeanied View Post
    I wonder what tece is trying to grow and where?

    I understand that some rootstocks - such as M116 - are propagated in this way, as it's quicker than stooling/layering if the rootstock is a bit slow to root.

    (slow rooting and more difficult propagation is why M116 never found success until lots of the commercial MM106's began to die from root diseases; a replacement rootstock with better disease resistance was urgently needed and M116 was too slow to propagate in the traditional way).

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    Last edited by FB.; 03-07-2012 at 07:32 PM.
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  6. #14
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    Here you go - a link to Frank P Matthews website, which mentions M116:

    Rootstocks : Background and History - Trees for Life - Frank P. Matthews Ltd.
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    Quote Originally Posted by FB. View Post
    To save HMSS from having to waste too much time, my registration number on the Health Professions Council website is BS40157 (Biomedical Scientist).
    SWMBO is also on there FB & is also a Biomedical Scientist
    He who smiles in the face of adversity,has already decided who to blame

    Artificial intelligence is no match for natural stupidity

    Merry Christmad everybody

  8. #16
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    Quote Originally Posted by bearded bloke View Post
    SWMBO is also on there FB & is also a Biomedical Scientist

    Yes, my SWMBO is also on there.

    However, although I am on the register, I have since chosen to move on to other things which I enjoy more. I will shortly be requesting removal from the register, as I don't see myself going back.
    I enjoyed the work and doing some good by identifying people's problems (including participating in the on-call/emergency service at night), but I hated the watering-down on new staff's training and qualification requirements, and the bureacracy which gradually crept in during the last government's term in office.
    But that's a subject for discussion on another board at another time.
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